Hochschule Reutlingen - Real-Time-PCR-System (qPCR-System)

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1. Subject of procurement A plate-based qPCR system with at least 96 reaction positions is to be procured for quantitative analysis of nucleic acids. Systems in 96-well or 384-well format are permissible; a 384-well format is not a minimum requirement. 2. Scope of application The system is used for molecular biology applications, in particular for the analysis of gene expression, for the quantification of nucleic acids, and for standardized qPCR-based investigations in the field of biomedical research and genomics. 3. Functional requirements The system must in particular be suitable for: - Real-Time PCR measurements - quantitative gene expression analyses (RT-qPCR) - absolute and relative quantification of nucleic acids - melting curve analyses - multiplex analyses - genotyping and copy number variation (CNV) applications - evaluation and documentation of measurement results 4. Basic technical requirements A system is required with the following basic properties: - thermocycler unit suitable for the intended qPCR applications - at least 96 reaction positions per run; 96-well and 384-well systems are permissible - suitable optical detection system - manufacturer-validated reaction…

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